Anti-Groen Fluorescent Proteïne (GFP) Antilichaam

Green Fluorescent Protein (GFP) is a naturally fluorescent protein originally derived from jellyfish. GFP has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors which have become useful and ubiquitous tools in transgenic experiments. Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions.

Our Anti-Green Fluorescent Protein (GFP) chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects n/a Green Fluorescent Protein (GFP), and is mixture of IgY fraction and affinity-purified antibodies. It is great for use in ELISA, IHC, ICC, WB.

Recombinant GFP expressed in Escherichia coli

Green Fluorescent Protein (GFP)

Primary Antibody

ELISA, IHC, ICC, WB

Anti-Tag Antibodies

10 mg/mL

Mixture of IgY fraction and affinity-purified antibodies

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.

27 kDa

Ice packs

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Chickens were immunized with recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using GFP conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (30 μg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.

1:2000-1:5000

1:5000-1:10000

1:2000-1:5000

Antibodies were analyzed by western blot analysis (1:5000 dilution) and immunohistochemistry (1:500 dilution) using transgenic mice expressing the GFP gene product. Western blots were performed using BlokHen® (Aves Labs) as the blocking reagent, and HRP-labeled goat anti-chicken antibodies (Aves Labs, Cat. #H-1004) as the detection reagent. Immunohistochemistry used tetramethyl rhodamine-labeled anti-chicken IgY.

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Liquid

Unconjugated

AB_10000240